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Reference (Technical Reports) ”Touchi” extract

Efficacy and safety of Touchi Extract, an α-glucosidase inhibitor derived from fermented soybeans, in non-insulin-dependent diabetic mellitus

■ Fujita Hiroyuki, Yamagami Tomohide, Ohshima Kazunori
Research and Development Department, Nippon Supplement, Inc., kita-ku, Osaka, 531-0076, Japan
Ohshima Clinic, Ibaraki, Osaka, 567-0829, Japan
■ Journal of Nutritional Biochemistry 12 (2001) 351

<< Abstract >>
The water-extracted Touchi, a traditional Chinese food, exerted a strong inhibitory activity against rat intestinal α-glucosidase in foodstuffs. In borderline and developed diabetic subjects, 0.3g of Touchi-extract (TE) significantly inhibited postprandial blood glucose levels, For confirmation of safety, 9 healthy subjects were given 1g of TE before every meal (3g/day) for 12 weeks. None indicated changes in hematological and relevant biochemical parameters, body weight or BMI. In a non-comparative study, 18 type-2diabetic patients ingested 0.3g of TE before every meal (0.9g/day) for 6 months (mo). Blood glucose (meam;9.31±0.71mmol/L) and HbA1c (10.24±0.58%) levels gradually decreased, and significant effects were elicited on the blood glucose levels (8.61±0.66mmol/; p<0.01) after 6 mo and HbA1c after3 (9.13±0.43%;p<0.05) and 6 mo (8.96±0.30%; p<0.05) post-ingestion of TE. Indexes for serum lipids and total cholesterol level revealed moderate decreases with a slight increase in the high-density lipoprotein (HDL) level after TE ingestion. However, triglyceride (TG) levels significantly decreased at 3 (p<0.05) and 6 mo (p<0.01)　post- ingestion of TE. In this study, other biochemical parameters were not affected in any of the patients, and no one complained of any side-effects or abdominal distension. TE, exhibitingα-glucosidase inhibitory activity, demonstrated an anti-hyperglicemic effect and may prove useful for improving glycemic control in patients suffering from non-insulin-dependent diabetes mellitus.

Fermented Soybean-Derived Water-Soluble Touchi Extract Inhibits α-Glucosidase and Is Antiglycemic in Rats and Humans after Single Oral Treatments

■ Hiroyuki Fujita, Tomohide Yamagami and Kazunori Ohshima
Research and Development Department, Nippon Supplement, Inc.,
Osaka, 531-0076, Japan and Ohshima Clinic, Ibaraki, Osaka, 567-0829, Japan
■ J.Nutr. 131: 1211, 2001.

<< Abstract >>
A water-soluble extract of Touch, a traditional Chinese food, was found to exert a strong inhibitory activity against rat intestinal α-glucosidase. We orally administered sucrose (2g/kg) with or without Touchi extaract (TE) to normal rats at 100 and 500 mg/kg. Postprandial increases in blood glucose levels at 30 and 60 min after the administration of TE were significantly depressed compared with controls. In humans, eight borderline diabetic subjects were administered 0.1-10.0 g TE before sucrose loading (75g). TE decreased the glycemic response dose dependently after sucrose loading. Compared with the area under the curve of the postprandial rise in blood glucose with various doses, TE elicited a significant antiglycemic effect at a minimum effective dose of 0.3 g. In addition, when four diabetics were administered 0.3 g TE before eating 200g of cooked rice, the postprandial increases in blood glucose and mean insulin levels were significantly depressed at 60 and 120min. respectively, after ingestion compared with levels when no TE was administered. TE, which exhibits α-glucosidase inhibitory activity, demonstrated an antihyperglycemic effect and may have potential use in the management or patients with non-insulin-dependent diabetic mellitus.

Fermented soybean-derived Touchi-extract with anti-diabetic effect via α-glucosidase inhibitory action in a long-term administration study with KKAy mice

■ Hiroyuki Fujita, Tomohide Yamagami
Research and Development, Nippon Supplement Inc.,
■ Life Science 70 (2001) 219

<< Abstract >>
A water-soluble Touchi-extract (TE) exerts a potent inhibitory activity against rat intestinal α-glucosidase in foodstuffs, and elicited anti-glycemic effects in rats and humans with single-bolus oral administration, In the present study, genetically modified diabetic model KKAy mice were used to examine the long-term effects of TE. Eight-week-old male KKAy mice were fed with CE-2 chow containing 0.08 and 0.4% of TE for 60 days. In the latter group, fasting blood glucose levels decreased (6.68±0.41 mmol/L) significantly (p<0.05) after a 60-day ingestion period compared with controls (8.75±0.54 mmol/L). Moreover, postprandial blood glucose levels were also significantly reduced (16.79±2.28mmol/L;P<0.01) after ingesting TE for only 30 days compared with controls (28.49±0.59mmol/L). On oral TE treatment for 60 days, postprandial increase in the blood glucose level after oral loading of sucrose (2g/kg) at 30 (p<0.05) and 60(P<0.01) min were significantly depressed compared with controls. Indexes for serum lipids; viz., total cholesterol (p<0.05) and triglyceride (p<0.01) levels significantly decreased after TE ingestion. Indexes for hepatic functions, such as glutamic-oxaloacetic transaminase (p<0.01), glutamic-pyruvic transaminase and gamma-glutamy1 transpeptide levels, were similarly suppressed. Organ weights of the heart, kidney, jejunum, liver and spleen increased in control KKAy mice due to hyperinsulinemia. Interestingly, the respective organ weights decreased (p<0.05,0.01) and jejunum length was reduced (P<0.05) significantly in the TE-treated groups. All in all, TE demonstrated an anti-hyperglycemic effect and may have potential use in the management of non-insulin-dependent diabetic mellitus.

Long-Term Ingestion of a Fermented Soybean-Derived Touchi-Extract withα-Glucosidase Inhibitory Activity Is Safe and Effective in Humans with Borderline and Mild Type-2 Diabetes
■ Hiroyuki Fujita, Tomohide Yamagami and Kazunori Ohshima*
Research and Development Department, Nippon Supplement, Incorporated., Kita-ku, Osaka, 531-0076,Japan and *Ohshima Clinic, Ibaraki,Osaka,567-0829,Japan
■ J. Nutr. 131 : 2105, 2001

<< Abstract >>
Water-extracted Touchi, a traditional Chinese food, exerts a strong inhibitory activity against rat intestinal α-glucosidase in foodstuffs, and Touchi-extract (TE) has been shown to have an antihyperglycemic effects in rats and humans after a single oral administration.　In the present complementary study, the effects of the powdered Houji-tea with or without (placebo) TE, a formula designed to enhance good compliance, were monitored in a 3-mo double-blind randomized group comparison study with placebo controls in humans with borderline and mild type-2 diabetes (n=36). All subjects ingested Houji-tea with or without 0.3g of TE before each of three meals per day for 3 mo. In the TE group, initial fasting blood glucose (6.9±0.1mmol/L) and glycated hemoglobin (HbA1c;6.1±0.1%) levels gradually decreased; fasting blood glucose decreased significantly after 3 mo (6.4±0.3mmol/L;P<0.05) as did HbA1c (5.6 ±0.2%;P<0.01) levels at 2 mo postingestion of TE and thereafter. In contrast, fasting blood glucose and HbA1c levels did not charge in any the placebo group. In this study, other biochemical variables were not affected in any of the subjects, and no one complained of any side effects or abdominal distension. Moreover, there was no deterioration as assessed by fasting blood glucose and HbA1c levels after withdrawal of TE ingestion. Thus, the α-glucosidase inhibitory TE demonstrated an antihyperglycemic effect and may prove useful for improving glycemic control in subjects suffering from borderline and type-2 diabetes mellitus.

Touchi Extract -Reference Report-

1.
Absence of mutagenicity, genotoxicity, and subchronic oral toxicity of Touchi extract.
Int. J. Toxycol. 26, 465-473 (2007).
H. Fujita, T. Yamagami:

2.
Effect of Touchi-extract on blood lipids in hypertriglyceridemic subjects and Sprague-dawley rats.
Nutrition Research. 25, 681-692 (2005).
H. Fujita, T. Yamagami and K. Ohshima

3.
Long-term ingestion of Touchi-extract, an α-glucosidase inhibitor, by borderline and mild type-2 diabetic subjects is safe and significantly reduces blood glucose.
Nutrition Research. 23, 713-722 (2003).
H. Fujita, T. Yamagami and K. Ohshima

4.
Fermented soybean-derived touchi extract inhibits α-glucosidase and in antiglycemic rats and humans after single oral treatments:
J. Nutri., 131, 121-124 (2001)
H. Fujita, T. Yamagami and K. Ohshima

5.
Fermented soybean-derived touchi-extract with anti-diabetic effect via α-glucosidase inhibitory action in a along-term administration study with KKAy mices:
Life Sci., 70, 219-227 (2001)
H. Fujita and T. Yamagami:

6.
Efficacy and safety of touchi extract, α-glucosidase inhibitor derived from fermented soybeans, in non-insulin-dependent diabetic mellitus:
J. Nutri. Biochem., 12, 351-356 (2001)
H. Fujita, T. Yamagami and K. Ohshima:

7.
Efficacy and safety of long-term ingestion of a fermented soybean-derived touchi-extract with an α-glucosidase inhibitory action in border line and mild type-2 diabetic human subjects:
J. Nutri., 131, 2105-2108 (2001)
H. Fujita, T. Yamagami and K. Ohshima:

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